There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy, and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. So the exploitation of various plant materials for the biosynthesis of nanoparticles is considered a green technology because it does not involve any harmful chemicals. The present study reported the green synthesis of silver nanoparticles using Acacia catechu leaves. Green synthesis of silver oxide nanoparticles (AgONps) was carried out using the aqueous extract of Acacia catechu leaves. The UV- Vis spectrum was recorded to monitor the formation of the nanoparticles, which exhibited a blue shifted absorption peak at 490 nm. The morphology structure and stability of the synthesized AgO nanoparticles were studied using scanning electron microscope (SEM). Further these biologically synthesized nanoparticles exhibited antibacterial activity against E.coli and Staphylococcus aureus. We cheked for anti bacterial activity which were found to be exhibiting