The development of bacterial resistance to presently available antibiotics has necessitated the need to search for new antibacterial agents. Gram positive bacteria such as Staphylococcus aureus are mainly responsible for post-operative wound infections, toxic shock syndrome, endocarditis, osteomyelitis and food poisoning. Gram negative bacterium such as Escherichia coli is present in human intestine and causes lower urinary tract infection, coleocystis or septicaemia. Multiple drug resistance in human pathogenic microorganisms has been developed due to indiscriminate use of commercial antimicrobial drugs commonly used in the treatment of infectious diseases. The development of antibiotic resistance is multi factorial, including the specific nature of the relationship of bacteria to antibiotics, the usage of antibacterial agent, host characteristics and environmental factors. This situation has forced scientists to search for new antimicrobial substances from various sources as novel antimicrobial chemotherapeutic agents, but the cost production of synthetic drugs is high and they produce adverse effects compared to plant derived drugs.