International Research journal of Management Science and Technology

  ISSN 2250 - 1959 (online) ISSN 2348 - 9367 (Print) New DOI : 10.32804/IRJMST

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EFFECT OF CRY1AC TOXIN FROM BACILLUS THURINGIENSIS SUBSP. KURSTAKI ON THE WHOLE CELL PROTEIN PROFILE OF TWO NIFH CONTAINING SOIL BACTERIA

    1 Author(s):  RITU MISHRA

Vol -  6, Issue- 1 ,         Page(s) : 115 - 136  (2015 ) DOI : https://doi.org/10.32804/IRJMST

Abstract

Ridge area of Delhi University, being rich in vegetation possesses rich and efficient nitrogen flux between environment and soil by virtue of its rich and vegetation. Microorganisms in this area have not been previously exposed to Bt-endotoxin. A total of 22 strains of bacteria were isolated by the process of enrichment and isolation on nitrogen free medium. Out of these 22 strains, two were selected by their partial identification through 16S rRNA phylogeny. Growth characteristics were studied for two strains viz. tempetrature and pH requirement for growth. RM3 showed 99% similarity with Sphingobium sp. and RM6 showed 99% similarity with Novosphingobium sp. The Bt-endotoxin was purified from Bacillus thuringiensis subsp. Kurstaki. Bioassay of isolated toxin was done on the larvae of Helicoverpa armigera to check their mortality and effectivity, and to calculate LC50 of endotoxin. The integrity of protein was checked on 10% SDS-PAGE. The protein profiles of bacteria were compared in absence and in presence of two different condition of Cry1Ac endotoxin. Different protein profile was observed for the different concentration of toxin for the same strain.

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