Abstract The researcher aimed towards comparing the Physiological fitness between under graduate and Post graduate females of Delhi University. A total numbers of three hundred (300) females from Delhi University, were selected randomly, their age ranged 17-23 years. Breath holding time was recorded in second and Vo2 Max was measured with Nomo-gram for the study. To find out the overall significant difference of mean for specific physiological fitness components of the under graduate and post graduate females “t” test was applied. The level of significance was set at 0.05. The analysis of the result revealed that, there was no significance difference found between both groups of undergraduate and postgraduate females but undergraduate players were superior in the Breath holding time capacity test as compared to the postgraduate females. In other items of Vo2 Max tests, there was a significance difference between undergraduate and postgraduate females, so undergraduate females were superior in the oxygen consumption and had better as compared to the postgraduate females from Delhi University.