The present study is concerned with the investigation of relationship among gender, spirituality and locus of control. It is often observed that in enhancing and improving quality of life spirituality and religion play crucial role. For this research 60 male and 60 female young adults were selected randomly. With the help of Spirituality Scale and Locus of Control Scale data was collected. The results of this study showed that gender is not a significant factor in determining the locus of control orientations, but it is in determining the spirituality level of young adults. The level of spirituality also was found to play mildly significant role in locus of control orientation, as people with high spirituality tend to show little higher internal locus of control in comparison of those with low spirituality level.